Table 2.
Average intrinsic parameters for layer 5 cell types recorded by the autonomous autopatcher
| Nonbursting | Bursting | Putative SOM Interneuron | Putative PV Interneuron | Statistical Significance | |
|---|---|---|---|---|---|
| Intrinsic property sample size | n = 14 | n = 21 | n = 1 | n = 1 | |
| Depth from pia, µm | 556 ± 12.5 | 532 ± 10.5 | 562 | 678 | B-pPV |
| Average resting voltage, mV | −72.2 ± 2.1 | −68.5 ± 2.1 | −56.5 | −61.2 | |
| Slope of membrane sag vs. Iinj, mV/pA | −0.002 ± 0.004 | −0.013 ± 0.018 | 0.049 | −0.0047 | NB-pSOM, B-pSOM |
| Membrane resistance, MΩ | 137 ± 6.73 | 138 ± 5.86 | 101 | 51.7 | |
| Spike threshold, mV | −35.18 ± 2.15 | −37.54 ± 1.01 | −35.29 | −36.78 | |
| Spike half-width, ms | 0.76 ± 0.05 | 0.83 ± 0.03 | 0.40 | 0.20 | B-pPV |
| Afterhyperpolarization, mV | 8.92 ± 1.49 | 3.77 ± 0.67 | 12.70 | 10.20 | NB-B |
| Recordings >5 min in duration | n = 11 | n = 16 | n = 1 | n = 1 | |
| Average spontaneous firing rate, Hz | 0.22 ± 0.12 | 0.94 ± 0.15 | 0.04 | 32.5 | NB-B, NB-pPV, B-pPV |
| Visual response sample size | n = 5 | n = 9 | n = 1 | n = 1 | |
| Average spike orientation tuning index (1-CirVar) | 0.447 ± 0.047 | 1.00 ± 0.40 | 0.75 | 0.27 | |
| Average spike direction tuning index (1-DirCirVar) | 0.30 ± 0.09 | 0.62 ± 0.17 | 0.93 | 0.14 |
Values are expressed as means ± SE. For the visual response metrics, the subset of cells with sufficient visual stimulus replicates were analyzed (n = 5: Nonbursting, n = 9: Bursting, n = 1: putative SOM+, n = 1: putative PV+). Significance column indicates significant difference between cell types shown (P values and test description are listed in Supplemental Table S1 available at https://doi.org/10.6084/m9.figshare.7946504.v2). PV, parvalbumin; SOM, somatostatin.