Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 5;12:5335–5354. doi: 10.2147/OTT.S198750

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Shi et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

siRNA-mediated downregulation of GATA1 suppresses invasion, proliferation and migration and promotes apoptosis of CCA stem cells through inactivation of the PI3K/AKT pathway. Stem cells from QBC-939 cells were treated with GATA1, si-GATA1, LY294002, si-GATA1+LY294002. (A and B) Protein bands and expression of GATA1 as well as the extent of PI3K, AKT and mTOR phosphorylation in stem cells from QBC-939 cells measured by Western blot analysis; (C) cell proliferation rate at 24 hrs, 48 hrs and 72 hrs in stem cells from QBC-939 cells assessed by CCK-8; (D and E) cell migration ability of stem cells from QBC-939 cells detected by scratch test (×40); (F and G), cell invasion ability of stem cells from QBC-939 cells evaluated by Transwell assay (×200); (H and I) cell apoptosis of stem cells from QBC-939 cells examined by Annexin V/PI double staining; *p<0.05 vs the blank and NC groups; #p<0.05 vs the si-GATA1 group; &p<0.05 vs the LY294002 group. Measurement data were expressed as means±standard deviation. Comparisons between multiple groups were assessed by one-way ANOVA followed by Tukey post hoc test.