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. 2019 Jul 5;12:5335–5354. doi: 10.2147/OTT.S198750

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© 2019 Shi et al.

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siRNA-mediated downregulation of GATA1 drives the primary cilium expression. (A) The primary cilium on the surface of CCA stem cells stained with red fluorescence observed by fluorescence staining, the morphology of the primary cilium was observed under different intervention conditions and nucleus was stained with blue by DAPI solution (×400); (B) the expression of the primary cilium in response to the treatment of GATA1, si-GATA1, LY294002 and si-GATA1+LY294002; *p<0.05 vs the blank and NC groups; #p<0.05 vs the si-GATA1 and LY294002 groups. Measurement data were expressed as means±standard deviation. Comparisons between multiple groups were assessed by one-way ANOVA followed by Tukey post hoc test.