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. 2019 May 31;20(7):2587–2597. doi: 10.1021/acs.biomac.9b00385

Figure 3.

Figure 3

Design of the PIC with new allyl monomers in the first block that can be further derivatized with the NITEC reaction. (a) Structure of the allyl monomer. (b) Reaction mechanism of the NITEC reaction. The fluorescent pyrazoline is depicted in red. (c) General structure of PIC5a–e. The part in red depicts the first block containing allyl functionality. (d) False color STORM images of PIC5b that was labeled with Alexa Fluor 647 (yellow) in the second block and Alexa Fluor 488 (blue) in the first block. Each 5 pixel cross represents the localization of a single dye with an accuracy of 12 nm (Alexa Fluor 647) or 20 nm (Alexa Fluor 488). The scale bar represents 50 nm.