Figure 11.

Bryostatin 1 induces Munc13-1 expression in primary hippocampal neurons. Primary hippocampal neurons were treated with either 0.1 or 0.5 μM bryostatin 1 for 24 h. (A) Double-label immunocytochemistry of Tuj-1 (green) and Munc13-1 (red) in primary hippocampal neurons. Nuclear staining, present only in the merged images, is colored blue. (B) Quantification of the mean intensity of Munc13-1 expression in the cytosol of primary hippocampal neurons. Six different regions from each coverslip per experimental group were imaged, and 10–20 cells from each field were counted. Data are means ± SEM of two or three independently performed experiments. ***P < 0.001, and **P < 0.01. Data were analyzed by one-way ANOVA with Tukey’s multiple-comparison test.