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. Author manuscript; available in PMC: 2019 Jul 11.
Published in final edited form as: Appl Opt. 2019 Mar 20;58(9):2248–2255. doi: 10.1364/AO.58.002248

Fig 1.

Fig 1.

Schematic of the sSMLM experimental system. The fluorophores are excited, and the emitted light is collected by an objective lens and passing through the dichroic mirror, tube lens, mirror and an entrance slit sequentially. Then the light passes through a transmission grating to be split into zeroth-order and first-order light, which provide the spatial and spectral information, respectively, and then two imaging lenses. The inset illustrates the zeroth-order (spatial) and first-order (spectral) images captured by the EMCCD.