Table 3.
Stability study data of APO-loaded COS-NPs (F4) after storage at refrigerated (4±1°C) and room (25±2°C/60±5% RH) temperatures
| Storage time | Evaluation parameters | |||||||
|---|---|---|---|---|---|---|---|---|
| Refrigerated temperature (4±1°C) | Room temperature (25±2°C/60±5% RH) | |||||||
| Particle size (nm) | PDI | ZP (mV) | Drug retention (%) | Particle size (nm) | PDI | ZP (mV) | Drug retention (%) | |
| Initial | 436.20±24.45 | 0.390±0.07 | +38.20±1.47 | 100.00±0.00 | 436.20±24.45 | 0.390±0.07 | +38.20±1.47 | 100.00±0.00 |
| 1 month | 438.57±4.73 | 0.354±0.04 | +37.80±0.85 | 99.30±0.50 | 450.08±31.69 | 0.438±0.10 | +36.10±1.63 | 99.10±0.79 |
| 2 months | 440.50±9.30 | 0.254±0.02 | +37.14±1.97 | 98.08±1.69 | 461.90±25.64 | 0.483±0.04 | +35.30±1.70 | 97.23±2.66 |
| 3 months | 449.73±36.11 | 0.317±0.14 | +36.30±2.04 | 97.10±2.04 | 501.40±21.81 | 0.481±0.06 | +33.10±1.13* | 96.30±2.48 |
| 4 months | 477.07±35.29 | 0.410±0.17 | +35.10±1.56 | 96.90±2.44 | 511.60±26.61* | 0.505±0.08 | +31.14±0.41* | 96.10±3.12 |
| 5 months | 478.87±24.48 | 0.429±0.06 | +34.70±1.42 | 95.60±3.18 | 541.03±24.77* | 0.555±0.11 | +28.58±1.22* | 95.20±3.81 |
| 6 months | 489.33±27.74# | 0.487±0.05 | +33.90±1.26# | 94.80±3.69 | 567.08±28.49* | 0.521±0.10 | +27.30±0.85* | 92.11±4.57 |
Notes: Each value represents the mean ± SD (n=3). *Significant at p<0.05 monthly vs initial. #Significant at p<0.05 refrigerated temperature vs room temperature after 6 months.
Abbreviations: APO, apocynin; COS, chitosan oligosaccharide; NPs, nanoparticles; PDI, polydispersity index; RH, relative humidity; ZP, zeta potential.