Figure 7.

Activation of GPR30 blocks NMDA-elicited currents. A, Whole-cell patch-clamp recordings were performed to detect the direct effect of G1 on the NMDA-elicited currents; the recording was stable for at least 30 min (A1). A2–A6, Representative recordings of currents elicited by a 0.5 min superfusion of 50 μm NMDA at baseline and in the presence of G1 (A2), E2 (A3), pretreatment with of Ro25–6981 (A4), GPR30-shRNA transfection (A5), and PD98059 (A6) from the culture neurons. B, Either 1 nm E2 (n = 6) or 1 nm G1 (n = 8) depressed exogenous NMDA-elicited currents. *p < 0.05 compared with the control. C, Pretreatment with Ro25–6981 (0.3 μm) totally blocked the effects of G1 on NMDA-elicited currents (n = 7). D, GPR30-shRNA transfection blocked the effects of G1 on NMDA-elicited currents (n = 8). E, PD98059 (25 μm) blocked the effects of G1 on NMDA-elicited currents (n = 6).