Figure 8.

Depression of NR2B subunit phosphorylation by GPR30. Cells were pretreated with G1 (1 nm) for 15 min and then cotreated with NMDA (200 μm) and glycine (20 μm) for another 30 min. A, Six hours after NMDA treatment, Western blot analysis was performed using antibodies against NR1, NR2A, and NR2B. B, GPR30 activation by G1 (1 nm, 45 min) did not affect the total NR1, NR2A, and NR2B expression. C, Western blot analysis was performed to detect the phosphorylated NR2B. D, GPR30 activation by G1 (1 nm, 45 min) depressed the levels of phosphorylated NR2B at Ser-1303 (p-Ser1303-NR2B), but not at (p-Tyr1472-NR2B). E, Detection of the membrane surface expression of NMDARs subunits. F, Only the membrane surface expression of NR2B-p-Ser1303 and not the total levels of NR2A and NR2B were affected by the G1 and NMDA treatments. The data were pooled from five independent experiments. ^$$p < 0.01 compared with control; *p < 0.05, **p < 0.01 compared with NMDA treatment alone.