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. 2012 Jun 6;32(23):8040–8052. doi: 10.1523/JNEUROSCI.4240-11.2012

Figure 2.

Figure 2.

Munc13 isoforms are differentially localized in the mouse retina. Single-plane confocal images of Munc13 labelings. A, Munc13-1, ub- and bMunc13-2, and Munc13-3 exhibited distinct punctate labeling patterns at the plexiform layers of the retina suggestive of a differential synaptic localization. B, C, ubMunc13-2 distribution extensively overlapped with that of the ribbon protein CtBP2 and of the vesicular glutamate transporter VGluT1, revealing its presence at ribbon synapses of the OPL (B) and the IPL (C). D, No such association was observed with bMunc13-2. E, A polyclonal antibody raised in guinea pig against bMunc13-2 yielded strictly the same labeling pattern as the rabbit polyclonal antibody used otherwise (left). Upon double labeling for bMunc13-2 and ubMunc13-2, no overlap was observed (middle and right panels). ONL, INL, Outer and inner nuclear layers; OPL, IPL, outer and inner plexiform layers; GCL, ganglion cell layer. Scale bars: A, E, 10 μm; B–D, 2 μm.