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. 2012 Jun 6;32(23):7771–7781. doi: 10.1523/JNEUROSCI.3286-11.2012

Figure 6.

Figure 6.

Noggin treatment of hESC colonies enhances the generation of NSCs but administration of Noggin to low-density neurosphere culture has no effect. When Noggin was administered as a “treatment” to hESC colonies grown to confluency in maintenance conditions, these cells went on to produce significantly more neurospheres than when Noggin was added directly to single cells in hESC colonies in neurosphere-forming minimal conditions (“Noggin in SFM”). Data are represented as a fold of control (neurosphere formation from single cells grown in SFM plus growth factors in the absence of Noggin). Two-way ANOVA shows a main effect of treatment (F(1,8) = 7.17, p = 0.03), n = 3. This result illustrates that Noggin-mediated neuralization takes place when cells are grown at high densities, rather than at low (clonal) densities, where paracrine signaling has ceased. Data are represented as mean ± SEM.