Figure 4.
The LV component of IPrl is abolished by 2-APB. A, Voltage-clamp recording of an oscillator neuron in the presence of TTX and 2-APB (200 μm). Application of Prl failed to induce an inward current. To the right, sharing its y-axis with the raw trace, are Gaussian fits of averaged (solid lines) holding current frequency distributions in control (green) and Prl (red). Raw data used to produce averages shown as dashed lines. B, Averaged voltage-clamp ramps recorded in the presence of 2-APB (200 μm; n = 9) acquired in control (green) and at the peak of the Prl response (red). C, Prl-induced current obtained by the digital subtraction of the traces displayed in B. Note the marked reduction in inward current throughout the LV but not HV range.