Skip to main content
. 2012 Jun 6;32(23):7791–7805. doi: 10.1523/JNEUROSCI.1309-12.2012

Figure 3.

Figure 3.

Temporal regulation of Neurog2 proneural activity by GSK3β. E12.5→E13.5 (A–K) and E14.5→E15.5 (L–V) neocortical electroporations with pCIG2 (A, B, L, M), Neurog2 (C, D, N, O), Neurog2+GSKCA (E, F), GSKDN (P, Q), and Neurog2+GSKDN (G, H, R, S), showing GFP+, transfected cells and NeuN immunolabeling. GFP+ cells that differentiated into NeuN+ neuronal cells are marked by arrows. The asterisks in photomicrographs mark nonspecific staining of blood vessels. I, T, The percentage of GFP+ transfected cells that expressed NeuN (I, T), Pax6 (J, U), and Tbr2 (K, V) was enumerated at E12.5 and E14.5. I, The asterisks indicate the following: *p < 0.001 versus pCIG2 or GSKCA and p < 0.05 versus GSKCA+Neurog2; **p < 0.01 versus GSKCA or pCIG2. T, The asterisks indicate the following: *p < 0.001 versus pCIG2, p < 0.01 versus Neurog2, p < 0.05 versus GSKDN+Neurog2. **p < 0.001 versus pCIG2 or Neurog2. The p values in J, K, U, and V were all relative to pCIG2 and are denoted as follows: *p < 0.05, **p < 0.001, ***p < 0.0001. O–T, E14.5→E15.5 Neurog2 transfection analyzed for the expression of GFP (O), Neurog2 (P), Neurod1 (Q), Bhlhe22 (R), Slc17a6 (S), and Tbr1 (T). U–Z, E14.5→E15.5 GSKDN transfection analyzed for the expression of GFP (U), GSK3β (V), Neurog2 (W), Bhlhe22 (X), Neurod6 (Y), and Tbr1 (Z). Error bars indicate SEM.