Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Aug 8;32(32):11050–11066. doi: 10.1523/JNEUROSCI.5664-11.2012

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2012 the authors 0270-6474/12/3211050-17$15.00/0

PMC Copyright notice

Figure 10. — Depletion of CDK5 resulted in defective neuronal migration, which was rescued by expression of GFP-Ndel1 (S251E) and GFP-Ndel1 (3E). a, Granular neurons were transfected with siRNA I against CDK5. Western blotting was performed 24 h after start of culture. CDK5 was clearly reduced by siRNA. Aurora-A and NDEL1 displayed similar expression levels after CDK5 knockdown, whereas Aurora-A and NDEL1 phosphorylated proteins were decreased after treatment of siRNA. Relative intensity of the bands of Western blotting is displayed at the bottom. Intensity was normalized with β-actin. Statistical examination was performed by unpaired Student's t test, with *p < 0.05 and **p < 0.01 (bottom panel). b, Immunocytochemistry using antibodies Aurora-A (b) or NDEL1 (c) revealed similar expression levels of these proteins, whereas levels of Aurora-A and NDEL1 phosphorylated proteins were decreased after treatment with siRNA I. d, Migration assay using granular neurons after depletion of CDK5. Hoechst images are shown at the bottom. e, The migration distance of each neuron 48 h after start of culture was binned. n is the number of neurons measured for each examination. f, Rescue experiments of siRNA knockdown of CDK5 after transfection of control GFP, GFP-Ndel1 (S251A), GFP-Ndel1 (S251E), mCherry-Ndel1 (3A), mCherry-Ndel1 (3E), and double transfection of GFP-Ndel1 (S251A), mCherry-Ndel1 (3A) and GFP-Ndel1 (S251E), mCherry-Ndel1 (3E). GFP images are shown in the top panels and Hoechst images are shown at the bottom. Expression of GFP-Ndel1 (S251A) or mCherry-Ndel1 (3A) had no effect on defective migration, whereas expression of GFP-Ndel1 (S251E) or mCherry-Ndel1 (3E) significantly improved defective migration due to siRNA knockdown of CDK5. g, The migration distance of each neuron 48 h after the start of culture was binned. n is the number of neurons measured for each examination. Expression of GFP-Ndel1 (S251E) or mCherry-Ndel1 (3E) significantly improved migration, which was characterized by a rightward shift. h, Statistical examination was performed to nuclear migration by an ANOVA followed by t test with correction: **p < 0.01. Note: Either GFP-Ndel1 (S251E) or mCherry-Ndel1 (3E) efficiently rescued defective migration, whereas double transfection of GFP-Ndel1 (S251E) and mCherry-Ndel1 (3E) did not result in further improvement.