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. 2012 Aug 8;32(32):11050–11066. doi: 10.1523/JNEUROSCI.5664-11.2012

Figure 11.

Figure 11.

Microtubule projections from MTOC was reduced by depletion of Aurora-A or the hypomorphic mutant of Ndel1. a, The projection speed of individual EB3-mCherry spots, and the calculated average extension length of microtubules per minute in the hypomorphic mutant. Experimental conditions are indicated at the bottom. The hypomorphic mutant of Ndel1 resulted in a reduction of EB3-mCherry emanation frequency. b, Extension frequency of EB3-mCherry spots from the MTOC, and the calculated average extension frequency of microtubules per minutes. Disruption of Ndel1 resulted in a reduction of EB3-mCherry extension speed. Reductions of emanation frequency and emanation speed were rescued by expression of GFP-Ndel1 (S251E), but not by GFP-Ndel1 (S251A). c, The projection speed of individual EB3-mCherry spots, and the average extension length of microtubules per minute was calculated. Experimental conditions are indicated at the bottom. Depletion of Aurora-A resulted in reduction of EB3-mCherry emanation frequency. d, Extension frequency of EB3-mCherry spots from the MTOC, and the calculated average extension frequency of microtubules per minute. Depletion of Aurora-A resulted in the reduction of EB3-mCherry extension speed. Reductions of emanation frequency and emanation speed were rescued by expression of GFP-Ndel1 (S251E), but not by GFP-Ndel1 (S251A). Statistical examination was performed by an ANOVA followed by t test with correction: **p < 0.01.