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. 2012 Aug 8;32(32):11050–11066. doi: 10.1523/JNEUROSCI.5664-11.2012

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Copyright © 2012 the authors 0270-6474/12/3211050-17$15.00/0

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Figure 4. — Rescue experiments of VX-680. a, Control GFP, GFP-Ndel1 (S251A), or GFP-Ndel1 (S251E) was transfected. GFP images (top), DIC images (middle), and Hoechst staining images (bottom) are shown. b, The migration distance of each neuron 24 h after start of culture was binned. n is the number of neurons measured for each examination. Expression of GFP-Ndel1 (S251A) had no effect on migration inhibited by VX-680, whereas expression of GFP-Ndel1 (S251E) significantly improved defective migration (a, b), which was characterized by rightward shift. c, Mean length of neurites (open bar) and nuclear position (solid bar) from the edge of central aggregation. Statistical examination was performed by an ANOVA followed by t test with correction: **p < 0.01.