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. 2012 Aug 8;32(32):11050–11066. doi: 10.1523/JNEUROSCI.5664-11.2012

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Copyright © 2012 the authors 0270-6474/12/3211050-17$15.00/0

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Figure 7. — In utero injection of Aurora-A KD resulted in defective neuronal migration. a, In utero injection of control GFP, GFP-Aurora-A, or GFP-Aurora-A KD into the E16.5 wild-type mouse neocortex by electroporation. GFP images are shown in the top panels. Counterstains of a neighboring section by DAPI are shown in the middle panels. The distribution of migrated neurons is shown in the bottom panels. Interestingly, expression of GFP-Aurora-A facilitated upward localization of GFP-positive neurons compared with control GFP, whereas expression of GFP-Aurora (KD) reduced upward localization of GFP-positive neurons. b, Rescue experiments with coexpression of either GFP-Ndel1 (S251A) or GFP-Ndel1 (S251E). Expression of GFP-Ndel1 (S251A) had no effect on defective migration after expression of mCherry-Aurora-A (KD), whereas expression of GFP-Ndel1 (S251E) significantly improved this defective migration. c, Distribution of GFP-Aurora (KD)-expressing cells within the brain. A fraction of GFP-Aurora (KD)-expressing cells remained within the ventricular zone. Expression of GFP-NDEL1(S251E) rescued the phenotype.