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. 2012 May 9;32(19):6587–6599. doi: 10.1523/JNEUROSCI.5317-11.2012

Figure 1.

Figure 1.

Expression and localization of LMTK1 in neurons. A, Immunoblots show expression of endogenous LMTK1, Rab11A, Cdk5, and p35 in a culture of mouse cortical neurons from DIV1 to DIV6. Cell lysates were collected at DIV1–6 and immunoblotted with antibodies against LMTK1, phospho-Ser34 of LMTK1 (pS34), Rab11A, Cdk5, and p35. Actin was used as the loading control. B, Immunofluorescence staining of primary cortical neurons with anti-pS34. Cortical neurons transfected with LMTK1-myc were doubly labeled with anti-myc (top) and anti-pS34 (middle). Merged images are shown in the bottom. The cell body and growth cone are indicated by the arrow and arrowhead, respectively. Higher-magnification views of the boxed areas are (left, right). C, Localization of LMTK1 to Rab11-positive recycling endosomes in neurons. LMTK1 was cotransfected into primary cortical neurons with EGFP-Rab5A, EGFP-Rab7, or EGFP-Rab11A. LMTK1 was visualized by immunostaining with anti-LMTK1 (left) 3 d after transfection. Rabs (middle). Merged images (right). Cell body (top). Axon (bottom). Arrows indicate colocalization of LMTK1 with Rabs in axons. N, Nucleus. Scale bars: B, 40 μm; C, 5 μm.