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. 2012 May 9;32(19):6587–6599. doi: 10.1523/JNEUROSCI.5317-11.2012

Figure 3.

Figure 3.

A kinase-negative mutant of LMTK1 promotes axonal outgrowth in cortical neurons. A, LMTK1-WT and the kinase-negative mutant LMTK1–D206V were individually cotransfected into cortical neurons with EGFP at DIV0, and axonal outgrowth was observed at DIV3. The control (Cont) image represents neurons transfected with EGFP alone. B, Axon length of EGFP-expressing neurons (mean ± SEM of 90 neurons from three independent experiments; *p < 0.01, Student's t test). C, The percentage of EGFP-expressing neurons with the indicated number of neurites (90 neurons from three independent experiments). D, Total length of neurites in LMTK1-expressing neurons (mean ± SEM of 90 neurons from three independent experiments; n.s., not significant; Student's t test). Scale bar, 20 μm.