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. 2012 Jan 18;32(3):761–774. doi: 10.1523/JNEUROSCI.3434-11.2012

Figure 1.

Figure 1.

Diagrams illustrating the surgery and brain areas that were sampled for neuron counts in HVC and NC in Experiment 1. a, Schematic illustration of the double labeling surgery. Green fluorescent cholera toxin B (Alexa-488-CTB, Invitrogen) is injected bilaterally into nucleus RA and red fluorescent cholera toxin B (Alexa-555-CTB, Invitrogen) is injected bilaterally into Area X. Birds are killed after 6 d to allow retrograde transport of the tracers back to the cell bodies in HVC. b, Dorsal view of adult zebra finch brain showing the placement and orientation of sections for neuron counts in HVC and NC. c, Frontal diagram in the plane of section shown in (b) representing part of one of the middle sections through HVC. The boxes show an example of the area included in a confocal Z-stack and used for neuron counts in HVC (red) and NC (blue). nXIIts, The tracheosyringeal portion of the nucleus of the twelfth cranial nerve; DLM, medial portion of the dorsolateral thalamus; HC, hippocampus; LAD, dorsal archopallial lamina.