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. 2012 Aug 15;32(33):11414–11423. doi: 10.1523/JNEUROSCI.6446-11.2012

Figure 4.

Figure 4.

Whole-cell voltage-clamp of HEK293 cells expressing recombinant TRPC3b or TRPC3c channels. A, Example of the larger currents produced by the TRPC3c transfected cells (currents activated by bath application of CCh (100 μm). The currents were blocked by pre-incubation with genistein (10 min; 200 μm). Example shows block of TRPC3c current; Vh = −40 mV; dashed lines indicate zero-current. B, Current/voltage relationships (I/Vs; mean ± SEM) for TRPC3b and TRPC3c (1 = control ramp before CCh; 2 = ramp during CCh response; 2–1 represents the isolated ITRPC3 I/V (trace 2-trace 1). The reversal potential (Erev) of ITRPC3 was close to 0 mV for both isoforms, indicating that the ion selectivity of the two channel isoforms was similarly nonselective. C, Mean peak whole-cell current responses for both isoforms of TRPC3 channels, genistein block for each, and control data (untransfected cells). ***p < 0.001; two-way ranked ANOVA, Holm–Sidak multiple pairwise comparisons).