Figure 2.

Knock-out of pannexin-1 attenuates the AD current. A, Immunoflourescence images of Panx1 expression in the hippocampus from vehicle-injected (left) and tamoxifen-injected (right) FlxPanx1-Cre mice. Top images are a 6 × 5 mosaic image taken at 20× magnification. Bottom panels are from the region indicated by the white box in the mosaic image. Scale bars: top, 300 μm; bottom, 100 μm. Note that Panx1 expression is lost in tamoxifen-treated animals, indicating knock-out. B, Mean (red line) ± SEM (gray region) whole-cell recordings of the anoxic depolarization from FlxPanx1-Cre mice treated with vehicle (left) or tamoxifen (right). C, Cumulative charge transfer during anoxia was significantly (*p < 0.05) reduced when Panx1 was knocked out. D, Quantitative analyses of the initial depolarization and residual current for Panx1 knock-out mice. Error bars are SEM.