Figure 4.

Src family kinase inhibition during anoxia blunts the anoxic inward current. A, Src family kinase activation during anoxia was assayed by Western blot analysis of phospho-Y416 levels. Phospho-Src was increased by anoxia and blocked by the Src inhibitor PP2 (10 μm), but the inactive analog, PP3 (10 μm), was ineffective. B, Quantitative analysis of relative density of Src Y416 in A compared with GAPDH levels in the same blots. The number of individual experiments (i.e., hippocampal slices) is indicated in each bar. Note that anoxia significantly (p < 0.05) increased phospho-Y416 levels, which was blocked by PP2 but not PP3. C, Mean ± SEM traces of the anoxic depolarization with intracellular PP3 (left) and PP2 (right). D, Quantitative summary of the initial depolarization (left) and residual current (right) with Src inhibition by PP2 included in the patch pipette. PP2 significantly reduced the residual currents compared with PP3 (p < 0.05). E, Src inhibition by PP2 decreased the cumulative charge transfer compared with PP3 (p < 0.05).