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. 2012 Sep 5;32(36):12506–12517. doi: 10.1523/JNEUROSCI.2306-12.2012

Figure 6.

Figure 6.

Similarities in the Ser845 phosphorylation of GluR1 subunit in vivo and in vitro. A, Western blot of synaptoneurosomes from half cortical hemisphere of C57BL/6J mice sleep deprived for 0, 2, or 6 h and sleep deprived for 6 h and let recovered for 2 or 4 h. A representative blot of Ser845-phosphorylated GluR1 and IgY loading control is shown. Quantitative analysis were performed with ImageJ and presented as the phosphorylated Ser845-GluR1 amount relative to IgY internal control (mean + SD of three mice per condition). B, Western blot of synaptoneurosomes from mature cortical cultures (11–13 DIV) stimulated with either distilled water (C, control) or the neurotransmitter cocktail and sampled after 1, 3, 6, or 24 h. A representative blot of Ser845-phosphorylated GluR1 and IgY loading control is shown. Quantitative analysis is presented as the phosphorylated Ser845-GluR1 amount relative to IgY internal control (mean + SD of three independent cultures). *Significant (p < 0.01) differences with the control condition (post hoc Tukey test).