Figure 10.

Upregulation of mGluR1 is induced by 5-HT_2AR via activation of PKC. As shown by Western blot analyses, 5-HT_2AR stimulation by spinal superfusion with agonist TCB-2 can upregulate mGluR1 protein in the spinal dorsal horn both in S2 and P3 fractions. For quantification, Western blot bands were normalized to S2 fraction band density in the control condition (superfusion with NaCl). Coadministration of PKC inhibitor chelerythrine markedly reduced TCB-2-induced upregulation of mGluR1. The asterisks indicate statistical significance at p < 0.01 at Student's t test. Ponceau staining was used to confirm equal protein loading. Error bars indicate SEM.