Figure 7.

Western blot analysis of the time course of upregulation of mGluR1 in synaptic fraction (P3) of dorsal horn homogenates in rats subjected to SNL, ipsilateral and contralateral to injury site. Relative density analyses from before injury revealed a significant ipsilateral increase of mGluR1 protein density 1 h after ligation, whereas increase in mGluR1 expression in dorsal horn contralateral to injury became significant at day 5 after ligation. The asterisks indicate statistical significance (p < 0.01) at Student's t test for density comparisons to before injury (total n was 20). As shown at the top, only the synaptic fraction but not cytoplasmic fraction S2 or crude synaptic vesicle fraction S3, was enriched in postsynaptic density protein PSD-95. Ponceau staining was used to confirm equal protein loading. Error bars indicate SEM.