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. 2012 Sep 26;32(39):13470–13487. doi: 10.1523/JNEUROSCI.5906-11.2012

Figure 3.

Figure 3.

Capsaicin-induced currents in L3 and L5 PCs in Gu-I. Aa, Ba, Sample traces of continuous recordings of inward currents (Icap) induced by 5 s puff application of 20 μm capsaicin and the current responses to the ramp pulses (Ab, Bb, top) applied before and during respective puff applications of capsaicin (blue bars), obtained in an L3 PC at 2 mm [Ca2+]o (A) and an L5 PC at 0.5 mm [Ca2+]o (B). Holding potential, −60 mV. Ab, Bb, Top, Positive ramp pulse from −100 to +50 mV for 1 s applied at the holding potential of −60 mV. Bottom, Superimposed enlarged traces of current responses seen during the respective time periods indicated with open horizontal bars in Aa (*0, *1 and *4) and Ba (*0, *1, *3 and *5). The blue bars indicate the duration and timing of puff applications of capsaicin. The current responses to the ramp pulse crossed each other at ∼0 mV (arrowheads). Ac, Bc, Mean (+SD) amplitudes of Icap obtained in response to the first through the third puff application of capsaicin, normalized to that obtained during the first puff application in L3 PCs at 0.5 mm [Ca2+]o (hollow columns: second, 1.9 ± 0.4; third, 2.6 ± 0.4; n = 5) and in a different set of L3 PCs at 2 mm [Ca2+]o (solid columns: second, 2.8 ± 0.8; third, 4.4 ± 1.5; n = 5) (Ac) and those in L5 PCs at 0.5 mm [Ca2+]o (hollow columns: 0.15 ± 0.12; 0.15 ± 0.11; n = 5) and in a different set of L5 PCs at 2 mm [Ca2+]o (solid columns: 0.06 ± 0.01; 0.07 ± 0.02; n = 5) (Bc). †p < 0.05; $p < 0.03; Πp < 0.05 (vs First); ΠΠp < 0.05 (vs Second). These symbols are the same as those applied in Ad and Bd. Ad, Bd, Mean (+SD) values of conductances (Gcap) obtained in response to the first through the third puff application of capsaicin, normalized to that obtained in the control conditions in L3 PCs at 0.5 mm [Ca2+]o (hollow columns: first, 1.2 ± 0.1; second, 1.3 ± 0.2; third, 1.4 ± 0.3; n = 5) and in the different set of L3 PCs at 2 mm [Ca2+]o (solid columns: first, 1.6 ± 0.3; second, 2.1 ± 0.3; third, 2.7 ± 0.3; n = 5) (Ad) and those in L5 PCs at 0.5 mm [Ca2+]o (hollow columns: 1.37 ± 0.22; 1.19 ± 0.09; 1.12 ± 0.05; n = 5) and in the different set of L5 PCs at 2 mm [Ca2+]o (solid columns: 1.15 ± 0.11; 1.03 ± 0.03; 1.01 ± 0.01; n = 5) (Bd).