Figure 7.

Regional specificity of P2X-mediated responses in the SOC. Whole-cell recordings from biocytin-labeled P9 neurons in the SOC. The labeling was visualized post hoc with Cy2-conjugated streptavidin. Under current-clamp, neurons show typical phasic responses to depolarizing current steps. Note that moderate responses to ATPγS application (150 ms) were recorded in the LSO and in the MNTB neuron, but not in the MSO neuron, the vitality of which was confirmed with glutamate application. The MSO neuron in the lower left is not from the slice shown in the center, but its respective position in the brain slice is indicated by the tip of the dashed line. The position of this neuron shows a good match with the axonal terminal field of the MNTB neuron on the right side. The current and voltage traces correspond to the adjacent neurons.