Figure 4.

Effect of NCX3 silencing on Ca²⁺ content in ER and [Ca²⁺]_i in NGF-differentiated PC-12 cells exposed to Aβ_1–42. A, Representative superimposed single-cell traces of the effect of 1 μm Tg on [Ca²⁺]_i in Ca²⁺-free (0 Ca²⁺; 1.5 mm EGTA) under control conditions (gray trace) and after 24 h Aβ_1–42 (black trace). B, Quantification of Aβ_1–42 effect on Tg-induced [Ca²⁺]_i release. Values are expressed as mean ± SEM of 3 independent experimental sessions (n = 50 for each group). C, Quantification of the effects of CB-DMB, several Ca²⁺ channel blockers, siControl, and siNCX3 after 24 h of 5 μm Aβ_1–42 on Tg-induced [Ca²⁺]_i release (n = 50 for each group). C, Inset, Quantification of the effects of Ca²⁺ channel blockers on NCX currents under control conditions and after 24 h 5 μm Aβ_1–42 (n = 16 for each group). Values are expressed as mean ± SEM of 3 independent experimental sessions. D, Quantification of the effect of Aβ_1–42 on [Ca²⁺]_i in the presence and in the absence of siNCX3. Values are expressed as percentage mean ± SEM of 3 independent experimental sessions (n = 60 for each experimental group). E, Quantification of the time-dependent effect of Aβ_1–42 on [Na⁺]_i. Values are expressed as percentage mean ± SEM of 3 independent experimental sessions (n = 50 for each experimental group). *p < 0.05 versus their untreated controls; **p < 0.05 versus Aβ_1–42 group; #p < 0.05 versus all groups.