Figure 7.
Effect of Aβ1–42 on calpain activation and formation of NCX3 proteolytic fragment in stably transfected BHK-NCX3 cells. A, Quantification of the time-dependent effect of 5 μm Aβ1–42 on [Ca2+]i. Values are expressed as percentage mean ± SEM of 3 independent experimental sessions (n = 40 for each experimental group). B, Representative Western blot and densitometric quantification of calpain activation under control conditions and after 6 and 12 h of Aβ1–42 exposure. Values are expressed as percentage mean ± SEM of 4 independent experimental sections. C, Representative Western blot and densitometric quantification of NCX3 protein expression and its proteolytic fragment under control conditions, after 12, 24, 48, and 72 h of 5 μm Aβ1–42 exposure. Values are expressed as percentage mean ± SEM of 7 independent experimental sections. D, Representative superimposed traces and quantification of INCX3 recorded under control conditions (gray trace) and after 5 μm Aβ1–42 exposure for 24 h (black trace). Values are expressed as mean ± SEM of 3 independent experimental sessions (n = 20 for each group). *p < 0.05 versus respective controls; **p < 0.05 versus 24 and 48 h of Aβ1–42 exposure.