Figure 2.

Capture of putative monosynaptic EPSCs elicited in response to reliable, long-duration photostimulation. A, B, Frequency histograms of the first (black) and last (gray) action potentials recorded in current-clamp mode in response to firing the calibrated laser pulses (100 ms; bold black line above the graphs) directly at the cell soma for both early (A) and late (B) time points (n = 46 and 59 photostimulation events, respectively). C, D, The number of EPSCs per 10 ms bin recorded under voltage-clamp conditions (in HDC ACSF) in response to firing the laser across the whole extent of the mapping grid. The arbitrary window used to pull out putative monosynaptic EPSCs (see text for full details) is highlighted by the gray histogram bars. E, F, A similar proportion of the recorded EPSCs was captured at both ages using our arbitrary criteria as revealed by the cumulative frequency plots.