Figure 5.

Effects of Z-3-HAC on the accumulation of H₂O₂, O₂⁻, and content of malondialdehyde (MDA) of the third fully expanded leaves in peanut seedlings under salinity stress. The seedlings were primed with distilled water or 200 μM Z-3-HAC twice. After priming, the seedlings were exposed to NaCl stress. At 7 days after the onset of salinity stress treatment, the leaves were excised, and histochemical staining of (A) H₂O₂ (DAB staining) and (B) O₂⁻ (NBT staining) were performed. Horizontal bar = 1 cm. Meanwhile, the leaves were collected for the determination of (C) H₂O₂ content, (D) MDA content, and (E) O₂⁻ content. Bars are the standard deviations (SD) of three independent replicates (n = 3). Error bars labels with different letters indicate significant differences at p < 0.05 between treatments according to Duncan’s test.