Figure 5.

Ablation of Tis21 in Patched1 heterozygous or wild-type mice reduces the differentiation of GCPs in the EGL. A, B, Representative confocal images of GCPs in the EGL of the four genotypes indicated; NeuroD1 expression in P7 mice (A) and NeuN expression in P14 mice (B). Sections are counterstained with Hoechst 33258 to visualize the EGL and the ML and IGL. Scale bar: 50 μm. C, D, Quantitative analysis of GCPs expressing NeuroD1 in the EGL of P7 and P14 mice (C) or expressing NeuN (D), represented as mean ± SEM percentage ratio between the number of NeuroD1⁺ or NeuN⁺ cells, respectively, and the total number of cells (labeled by Hoechst 33258) (n = 3 for each genotype). **p < 0.01 or ***p < 0.001, Student's t test.