Figure 6.

Ablation of Tis21 in Patched1 heterozygous or wild-type mice impairs the migration of GCPs from EGL to molecular and internal granular layers. A, B, Representative confocal images of GCPs migrating outside the EGL, identified as BrdU⁺ cells in Tis21-null and wild-type mice, in either Patched1 heterozygous or wild-type background, as indicated. P7 mice were injected with BrdU and analyzed after 42 h (A) or after 5 d (B). Sections are counterstained with Hoechst 33258 to visualize the ML and the IGL. Scale bars, 50 μm. C, D, Quantification of GCPs migrating from the EGL 42 h after BrdU injection in P7 mice (C), or after 5 d (D), represented as mean ± SEM percentage ratio of BrdU⁺ cells in the EGL, ML, or IGL to the total BrdU⁺ cells. Three mice per genotype were analyzed. *p < 0.05, or **p < 0.01, or ***p < 0.001, Student's t test.