Table 1.
Antibody specification
| Antigen | Immunogen | Manufacturer, species, mono- or polyclonal, catalog and lot no | Dilution used | |
|---|---|---|---|---|
| Primary antibodies | Calbindin-D28k | Calbindin-D28k purified from chicken gut was used as the immunogen | Sigma-Aldrich, mouse monoclonal, Cat. # C-8666, Lot # 058H4812 | 1:6000 |
| Calbindin-D28k | Synthetic peptide derived from amino acids 185-199 of human Calbindin-D-28K | AnaSpec, rabbit polyclonal, Cat. # 53283, Lot # GL141 | 1:4000 | |
| EphA4 | Purified, NS0-derived, recombinant mouse EphA4 extracellular domain | R&D Systems, goat polyclonal, Cat. # AF641, Lot # BVX0308091 | 1:1000 | |
| FoxP2 | Synthetic peptide derived from C-terminal amino acids of isoforms I, II, IV, and V of human FOXP2: REIEEEPLSEDLE | Everest Biotech, goat polyclonal, Cat. # EB05226, Lot # 160409 | 1:5000 for fluorescent, 1:10000 for DAB | |
| FoxP2 | A KLH-conjugated synthetic peptide between 664 and 693 amino acids from the C-terminal region of human FOXP2 | Abgent, rabbit polyclonal, Cat. # AP5753b, Lot # SA100916AA | 1:1000 | |
| Pcdh10 | The cytoplasmic domain of the OL-pc isoform (Aoki et al., 2003) | Millipore, rat monoclonal, clone 5G10, Cat. # MABT20, Lot # NRG1759424 | 1:1600 | |
| PLCβ4 | Amino acids 876-1115 of PLCβ4 of human origin | Santa Cruz Biotechnology, rabbit polyclonal, Cat. #sc-20760, Lot # A1508 | 1:150 | |
| RORα | Amino acids 196-260 mapping within an internal region of RORα2 of human origin | Santa Cruz Biotechnology, rabbit polyclonal, Cat. # sc-28612, Lot # I0909 | 1:100 | |
| Secondary antibodies | Anti-goat IgG, biotin-conjugated | Vector Laboratories, rabbit, Cat. # BA-5000 | 1:333 | |
| Anti-rabbit IgG, biotin-conjugated | Vector Laboratories, goat, Cat. # BA-1000 | 1:333 | ||
| Anti-mouse IgG, biotin-conjugated | Vector Laboratories, goat, Cat. # BA-9200 | 1:333 | ||
| Anti-goat IgG, fluorescein-conjugated | Jackson ImmunoResearch, donkey, Cat. # 705-095-147 | 1:333 | ||
| Anti-goat IgG, DyLight 488-conjugated | Jackson ImmunoResearch, donkey, Cat. # 705-485-147 | 1:400 | ||
| Anti-rabbit IgG, Texas Red-conjugated | Jackson ImmunoResearch, donkey, Cat. # 711-075-152 | 1:333 | ||
| Anti-rat IgG, DyLight 594-conjugated | Jackson ImmunoResearch, donkey, Cat. # 712-515-153 | 1:333 | ||
The data are based on manufacturer's data sheets and in some cases on communications with manufacturers.