Figure 7.

Nogo-A and NgR1 inhibition in SVZ-OB explants. A, B, Micrographs show Nogo-A⁺ cells (A) that express DCX in SVZ-OB explants at 1 d in vitro. C, Quantification of the decrease in the migration areas of 11C7 exposed explants versus controls (SVZ, ctrl vs 11C7, p = 0.012, n = 5; RMS, ctrl vs 11C7, p = 0.008, n = 4; OB, ctrl vs 11C7, p = 0.218, n = 4, paired t test). D, E, Examples of reduced neuroblast migration after 11C7 treatment. F, Analysis of neuroblast migration areas at increasing concentrations of 11C7 (50 ng/ml, p = 0.59; 100 ng/ml, p = 0.036; 0.5 μg/ml, p = 0.030; n = 3). G, Number of Ph3⁺ cells in control and 11C7-treated explants (SVZ, RMS, p > 0.05, n = 3). H, Quantification of the distance from the explant core of neuroblasts moving in chains in 11C7-treated or control explants. I, J, Representative images of control (I) and NgR1 antagonized (J) explants at 1 d in vitro. K, Quantification shows no changes in the migration areas in NgR1-antagonized explants compared with controls (anti-α-NgR vs ctrl, SVZ, p = 0.89; RMS, p = 0.79; OB, p = 0.52, n = 5; NEP1–40 vs ctrl, SVZ, p = 0.91; RMS, p = 0.67; OB, p = 0.61; n = 3). L, M, NgR1 antagonization does not change neuroblast migration areas when explants are plated on a feeder layer of RMS astrocytes (NEP1–40 vs ctrl, SVZ, 96.6 ± 7.2%, 100 ± 18.2%, p = 0.87; RMS, 117.2 ± 15.2%, 100 ± 10.7%, p = 0.52; n = 3). N, O, Quantification of the decrease in the migration areas during ROCK inhibition (J, SVZ, ctrl vs Y27632, p = 0.003; ctrl vs 11C7 + Y27632, p = 0.004; SVZ, Y27632 + 11C7 vs Y27632, p = 0.31, unpaired t test, n = 4; K, RMS, ctrl vs Y27632, p = 0.009; ctrl vs 11C7 + Y27632, p = 0.03, unpaired t test; RMS, Y27632 + 11C7 vs Y27632, p = 0.42; n = 4). P, Q, Neuroblast emigration from explants during ROCK blockade. Unpaired t test: *p < 0.05, **p < 0.01, ***p < 0.0001. Error bars indicate SEM. Scale bars: A, B, 50 μm; D, E, G–J, 100 μm; P, Q, 40 μm.