Figure 5.

Pharmacological inactivation of LH neurons inhibits PS. A, Hypnograms illustrating the organization of the sleep-waking cycle during the 16 h (between 10:00 A.M. and 2:00 A.M.) following an injection of NaCl, clonidine, or muscimol in the LH in a representative animal. A gray background differentiates the dark period. B–E, Quantitative analysis of sleep by 2 h blocks during the 16 h after muscimol (black lines), clonidine (black dashed lines), and NaCl (gray lines) application in LH. The quantities per 2 h blocks (B), the cumulated quantities across the 16 h (C), the number of episodes (D), and the mean duration of episodes (E) are given for PS and SWS. For all graphs, n = 8 animals per group, values are mean ± SEM and *p < 0.05 between muscimol and NaCl, ^#p < 0.05 between clonidine and NaCl.