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. 2012 Nov 21;32(47):16560–16573. doi: 10.1523/JNEUROSCI.1787-12.2012

Figure 5.

Figure 5.

Histological examination of the layered structures of Scyl1+/+ and Scyl1−/− cerebellum. A, Representative micrographs of H&E-stained sagittal sections of cerebellums obtained from 17-week-old Scyl1+/+ and Scyl1−/− mice. No gross deformation in the cerebellar structures was observed in Scyl1−/− mice. I–X, Cerebellar lobules. Scale bar, 1 mm. B, Representative micrographs of H&E-stained sections of 8-week-old Scyl1+/+ and Scyl1−/− mice showing the layered structures of the cerebellum. The layered structures of the cerebellum of Scyl1−/− were undistinguishable from Scyl1+/+ mice. gl, Granular layer; pcl, Purkinje cell layer; ml, molecular layer. Scale bar, 50 μm. C, Purkinje cell numbers in Scyl1+/+ and Scyl1−/− mice. Purkinje cells numbers were determined as described in Materials and Methods. For Purkinje cell counts, a total of 25 segments from three Scyl1+/+, 21 segments from three Scyl1−/−, and 14 segments from three Scyl1mdf/mdf male mice of 17 weeks of age were counted. Values are expressed as mean ± SEM. p > 0.05. D, E, Microglial activation in the white track of the cerebellar peduncle. Immunohistochemistry using antibodies against Iba1 (D) and Mac2 (E) on cerebellar sections of 8-week-old Scyl1+/+ (left) and Scyl1−/− (right) mice. Note the increased Iba1 and Mac2 staining in the cerebellar peduncle of Scyl1-deficient mice. Scale bar, 100 μm.