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. 2012 Feb 15;32(7):2357–2376. doi: 10.1523/JNEUROSCI.5243-11.2012

Table 1.

Identity, source, and characterization of antibodies

Molecule Code no. in original laboratory Host animal Developer Epitope, amino acid residues Protein concentration Optimal dilution FRL Characterization testsa
Reference
Immunohistochemistry Western blot FRL
GluA1-4 (panAMPAR) Anti-GluR1-4 (R3/BO A/P 30/08/01) Rabbit E. Molnár Mouse GluA1 724–781 Purified ab., 330 μg/ml 300× Brain, postembedding EM COS7 cells WT rat and mouse, GluA2/3 KO Nusser et al., 1998; Masugi-Tokita et al., 2007
vGluT2 Anti-DNPI-Gp (670 μg/ml) Guinea pig M. Watanabe Rat 559–582 Purified ab., 670 μg/ml 200× Brain, pre-embedding Brain WT rat Miyazaki, 2003; Kawamura et al., 2006; Miura et al., 2006; Masugi-Tokita et al., 2007
GLT1 (EAAT2) Anti-GLT-1 (0.96 mg/ml) Rabbit K. Tanaka Mouse 500–525 Purified ab., 960 μg/ml 300× Brain Brain No Yamada et al., 1998
GLAST (EAAT1) Anti-GLAST (1000) Rabbit M. Watanabe Mouse C-term 41aa Purified ab., 1 mg/ml 300× Brain Brain No Shibata et al., 1997; Yamada et al., 2000; Watase et al., 1998

aData are presented in cited publications.