Table 1.
Molecule | Code no. in original laboratory | Host animal | Developer | Epitope, amino acid residues | Protein concentration | Optimal dilution FRL | Characterization testsa |
Reference | ||
---|---|---|---|---|---|---|---|---|---|---|
Immunohistochemistry | Western blot | FRL | ||||||||
GluA1-4 (panAMPAR) | Anti-GluR1-4 (R3/BO A/P 30/08/01) | Rabbit | E. Molnár | Mouse GluA1 724–781 | Purified ab., 330 μg/ml | 300× | Brain, postembedding EM | COS7 cells | WT rat and mouse, GluA2/3 KO | Nusser et al., 1998; Masugi-Tokita et al., 2007 |
vGluT2 | Anti-DNPI-Gp (670 μg/ml) | Guinea pig | M. Watanabe | Rat 559–582 | Purified ab., 670 μg/ml | 200× | Brain, pre-embedding | Brain | WT rat | Miyazaki, 2003; Kawamura et al., 2006; Miura et al., 2006; Masugi-Tokita et al., 2007 |
GLT1 (EAAT2) | Anti-GLT-1 (0.96 mg/ml) | Rabbit | K. Tanaka | Mouse 500–525 | Purified ab., 960 μg/ml | 300× | Brain | Brain | No | Yamada et al., 1998 |
GLAST (EAAT1) | Anti-GLAST (1000) | Rabbit | M. Watanabe | Mouse C-term 41aa | Purified ab., 1 mg/ml | 300× | Brain | Brain | No | Shibata et al., 1997; Yamada et al., 2000; Watase et al., 1998 |
aData are presented in cited publications.