Figure 6.

ANI infusions that suppress hippocampal activity do not elicit hippocampal cell death. Expansion photomicrographs from a single histological section are shown from Fluoro Jade B-treated brain slices. The left inset panels show suprathreshold (positively stained) cells, while the right inset panels show the locations at which images were taken. A, Many positively stained (dead or dying) cells were observed in superficial cortical regions damaged by skull trephination and pial damage. However, no systematic staining was observed in either the contralateral HPC (B) or ipsilateral HPC (C). In this experiment, CSD RMS analysis at the level of SLM showed an 87.54% decrease in activity during SO and an 86.93% decrease during theta (across all probe channels this decrease was 78.02% during SO and 74.53% during theta) when assessed 30 min following ANI infusion (50 μg in 0.5 μl).