Table 3.
Inactivation parameters of Geeraerd et al. (2000) model for S. Typhimurium planktonic cells and biofilms after plasma activated liquid treatment.
| Cell type | Gas composition | Generation time | Population | Inactivation parameters |
RMSE | 1log reduction23 | ||
|---|---|---|---|---|---|---|---|---|
| 1log N0 (log [CFU/mL)]23 / 1log N0 [log(CFU/cm2)]23 | 1kmax (1/min)23 | 1log Nres [log(CFU/mL)]23 / 1logNres [log(CFU/cm2)]23 | ||||||
| Planktonic cells | 0% (v/v) O2 | 10 min | Total | 7.2 ± 0.01A | 0.011 ± 0.0061 | – | 0.1255 | ≈ 10.2 ± 0.01A |
| Uninjured | 7.2 ± 0.01A | 0.020 ± 0.0061 | – | 0.1276 | ≈ 10.3 ± 0.01A | |||
| 20 min | Total | 7.2 ± 0.31A | 21.686 ± 0.3231 | 3.5 ± 0.22 | 0.5676 | 23.7 ± 0.41B | ||
| Uninjured | 7.1 ± 0.31A | 12.159 ± 1.1451 | 3.3 ± 0.22 | 0.5250 | 23.8 ± 0.41B | |||
| 30 min | Total | 7.2 ± 0.61A | 17.659 inf | 1.0 ± 0.31 | 1.1470 | 26.2 ± 0.71C | ||
| Uninjured | 7.1 ± 0.51A | 7.592 inf | 1.2 ± 0.31 | 1.0927 | 25.9 ± 0.61C | |||
| 1% (v/v) O2 | 10 min | Total | 7.0 ± 0.31A | 0.463 ± 0.1862A | 5.3 ± 0.2C | 0.6393 | 11.7 ± 0.42A | |
| Uninjured | 7.1 ± 0.31A | 0.762 ± 0.2792A | 5.1 ± 0.2C | 0.6832 | 12.0 ± 0.42A | |||
| 20 min | Total | 7.2 ± 0.71A | 12.779 ± 8.6341A | 2.5 ± 0.41B | 1.3344 | 24.7 ± 0.81B | ||
| Uninjured | 7.2 ± 0.71A | 12.495 ± 1.0281B | 2.0 ± 0.41B | 1.3610 | 25.2 ± 0.81B | |||
| 30 min | Total | 7.1 ± 0.51A | 12.962 ± 1.995A | 1.5 ± 0.31A | 1.1517 | 25.6 ± 0.61B | ||
| Uninjured | 7.1 ± 0.51A | 22.667 ± 0.615B | 1.3 ± 0.31A | 1.0945 | 25.8 ± 0.61B | |||
| Biofilms | 0% (v/v) O2 | 10 min | Total | 6.4 ± 0.11AB | 7.650 inf | 5.1 ± 0.12C | 0.4905 | 21.3 ± 0.11A |
| Uninjured | 5.9 ± 0.21A | 10.683 inf | 4.3 ± 0.22B | 0.6235 | 21.6 ± 0.31A | |||
| 20 min | Total | 6.5 ± 0.11B | 10.441 ± 0.0941 | 4.8 ± 0.12B | 0.3836 | 11.7 ± 0.11B | ||
| Uninjured | 6.2 ± 0.11B | 10.585 ± 0.1451 | 4.1 ± 0.22B | 0.5457 | 12.1 ± 0.21B | |||
| 30 min | Total | 6.3 ± 0.11A | 12.753 inf | 3.8 ± 0.12A | 0.5534 | 12.5 ± 0.11C | ||
| Uninjured | 6.0 ± 0.11AB | 8.823 inf | 3.1 ± 0.12A | 0.5698 | 12.9 ± 0.11C | |||
| 1% (v/v) O2 | 10 min | Total | 6.3 ± 0.11A | 11.335 inf | 4.7 ± 0.11C | 0.5150 | 11.6 ± 0.12A | |
| Uninjured | 5.9 ± 0.21A | 6.925 inf | 3.8 ± 0.21C | 0.6264 | 12.1 ± 0.31A | |||
| 20 min | Total | 6.3 ± 0.11A | 11.269 ± 0.1582 | 3.1 ± 0.11B | 0.4751 | 13.2 ± 0.12B | ||
| Uninjured | 6.1 ± 0.11A | 11.560 ± 0.2022 | 2.4 ± 0.21B | 0.5521 | 13.7 ± 0.22B | |||
| 30 min | Total | 6.2 ± 0.11A | 11.403 ± 0.160 | 2.6 ± 0.11A | 0.5000 | 13.6 ± 0.12C | ||
| Uninjured | 5.9 ± 0.11A | 11.579 ± 0.174 | 2.0 ± 0.21A | 0.5330 | 13.9 ± 0.22B | |||
Plasma activated liquids were created using different cold atmospheric plasma gas compositions and generation times. Inactivation parameters are determined for both the total and the uninjured population. 1Effect cell mode: for each gas composition, generation time and population type, parameters of the Geeraerd et al. (2000) model bearing different superscripts (no numbers in common) are significantly different (P ≤ 0.05). 2Effect gas composition: for each cell mode, generation time and population type, parameters of the Geeraerd et al. (2000) model bearing different superscripts (no number in common) are significantly different (P ≤ 0.05). 3Effect generation time: for each cell mode, gas composition and population type, parameters of the Geeraerd et al. (2000) model bearing different subscripts (no uppercase letters in common) are significantly different (P ≤ 0.05).