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. 2019 Jul 4;10:1539. doi: 10.3389/fmicb.2019.01539

Table 4.

Inactivation parameters of Geeraerd et al. (2000) model for L. monocytogenes and S. Typhimurium planktonic cells and biofilms after treatment with plasma activated liquids of different ages.

Microorganism Cell type PAL age Population Inactivation parameters
RMSE 1log reduction2
1log N0 (log [CFU/mL)]2 / 1log N0 [log(CFU/cm2)]2 1kmax (1/min)2 1log Nres [log(CFU/mL)]2 / 1logNres [log(CFU/cm2)]2
Listeria monocytogenes Planktonic cells 0 days Total 6.8 ± 0.71 2.572 ± 0.8102 1.3 ± 0.4 1.3619 25.5 ± 0.82
Uninjured 6.5 ± 0.51 19.272 inf 1.2 ± 0.3 1.0609 25.3 ± 0.62
3 days Total 6.9 ± 0.01 10.001 ± 0.0051 0.0965 10.0 ± 0.01
Uninjured 6.9 ± 0.01 10.001 ± 0.005 0.1028 10.0 ± 0.01
10 days Total 6.9 ± 0.01 10.001 ± 0.0041 0.0742 10.0 ± 0.01
Uninjured 6.9 ± 0.01 10.001 ± 0.003 0.0656 10.0 ± 0.01
30 days Total 6.9 ± 0.01 10.002 ± 0.0041 0.0791 10.1 ± 0.01
Uninjured 6.9 ± 0.01 10.006 ± 0.004 0.0789 10.1 ± 0.01
Biofilms 0 days Total 7.3 ± 0.12 6.017 inf 4.3 ± 0.11 0.4363 13.0 ± 0.13
Uninjured 7.2 ± 0.12 6.022 inf 4.0 ± 0.11 0.5238 13.2 ± 0.13
3 days Total 7.3 ± 0.32 20.703 ± 0.230 5.4 ± 0.23 0.5604 21.9 ± 0.42
Uninjured 7.2 ± 0.32 20.704 ± 0.236 5.2 ± 0.23 0.6054 22.0 ± 0.42
10 days Total 6.8 ± 0.31 20.565 ± 0.187 4.7 ± 0.22 0.6262 22.1 ± 0.42
Uninjured 6.6 ± 0.31 20.548 ± 0.173 4.4 ± 0.22 0.6310 22.2 ± 0.42
30 days Total 6.9 ± 0.31,2 10.588 ± 0.579 5.9 ± 0.24 0.6504 21.0 ± 0.41
Uninjured 6.8 ± 0.31,2 20.511 ± 0.292 5.6 ± 0.24 0.5645 21.2 ± 0.41
Salmonella Typhimurium Planktonic cells 0 days Total 7.1 ± 0.51,2 12.962 ± 1.9952 1.5 ± 0.3 1.1517 25.6 ± 0.62
Uninjured 7.1 ± 0.51 22.667 ± 0.6152 1.3 ± 0.31 1.0945 25.8 ± 0.63
3 days Total 7.0 ± 0.11 10.015 ± 2.2131 7.0 ± 4.4 0.2320 10.0 ± 4.41
Uninjured 7.0 ± 0.21 10.117 ± 0.1031 6.1 ± 0.53 0.5028 10.9 ± 0.51
10 days Total 7.1 ± 0.11,2 10.086 ± 0.0111 0.3008 12.0 ± 0.11,2
Uninjured 7.1 ± 0.11 10.172 ± 0.0411 4.9 ± 0.52 0.4492 12.2 ± 0.52
30 days Total 7.5 ± 0.12 10.272 ± 0.1041,2 6.8 ± 0.1 0.2136 10.7 ± 0.11
Uninjured 7.5 ± 0.21 10.434 ± 0.2961 6.6 ± 0.13 0.4272 10.9 ± 0.21
Biofilms 0 days Total 6.2 ± 0.11,2 11.403 ± 0.1601 2.6 ± 0.11 0.5000 13.6 ± 0.13
Uninjured 5.9 ± 0.12,3 11.579 ± 0.1741 2.0 ± 0.21 0.5330 13.9 ± 0.22
3 days Total 6.5 ± 0.22 11.500 ± 1.7441 4.4 ± 0.14 0.4633 12.1 ± 0.21,2
Uninjured 6.2 ± 0.33 21.324 ± 0.3501 3.5 ± 0.13 0.5145 22.7 ± 0.31
10 days Total 5.9 ± 0.21 20.815 ± 0.2311 4.1 ± 0.13 0.4524 11.8 ± 0.21
Uninjured 5.7 ± 0.32 11.523 ± 1.1501 3.3 ± 0.22,3 0.5394 12.4 ± 0.41
30 days Total 6.2 ± 0.21,2 20.774 ± 0.1621 3.9 ± 0.12 0.4315 22.3 ± 0.22
Uninjured 5.2 ± 0.31 10.739 ± 0.1851 3.0 ± 0.22 0.5150 22.2 ± 0.41

Inactivation parameters are determined for both the total and the uninjured population. 1Effect cell mode: for each microorganism, PAL age and population type, parameters of the Geeraerd et al. (2000) model bearing different superscripts (no numbers in common) are significantly different (P ≤ 0.05). 2Effect PAL age: for each microorganism, cell mode population type, parameters of the Geeraerd et al. (2000) model bearing different superscripts (no number in common) are significantly different (P ≤ 0.05).