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. 2019 Jul 10;13:63. doi: 10.1186/s13036-019-0191-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Chromatographic results of the reactant ssDNA (a), and the phosphoramidated ssDNA conjugate (b). Column dimensions: XTerra® MS C18 2.5 μm, 4.6 mm × 50 mm. Ion pairing reagent: 0.1 M TEAA, pH 9.70 ± 0.05. Mobile phases: A; 5% acetonitrile in TEAA. B; 30% acetonitrile in TEAA. Gradient conditions: 90%A – 60%A in 16 min. 60–90% A in the next 14 min. Flowrate: 1.0 mL/min. Column and samples temperatures: 37 °C, and 33 °C. Injection volume: 100 μL. UV detector: 260 nm