Fig. 1.

Phenotypes of spermatogonia treated with MA2. a Effect of MA2 on the cellular m⁶A level. GC-1 cells were treated with 0, 40, 80 and 120 μmol/L of MA2 for 48 h. Cellular m⁶A level was analyzed via m⁶A dot blot. b Quantification of cellular m⁶A level through HPLC/MS-MS. Data were presented as the mean ± SEM, n = 3. *P < 0.05. c Images of the GC-1 spg cells. Cells were treated with 40, 80 and 120 μmol/L of MA2 or the carrier DMSO for 48 h in triplicates. The morphology of cells was photographed. Bar = 50 μm. d Dose-dependent effect of MA2 on cell viability. GC-1 cells were treated with MA2 for 48 h. Different lower-case letters denoted significant differences (P < 0.05). e Time-dependent effect of MA2 on cell viability. GC-1 cells were treated with 0, 40, 80 and 120 μmol/L MA2 in triplicates. Cell viability was detected via CCK-8 assay. Data were presented as the mean ± SEM, n = 3. *P < 0.05