Figure 3.

Overactivation of Notch reduces generation of new motor neurons and proliferation in the lesioned spinal cord. Spinal cross sections, centered around the central canal (asterisks in B, D), are shown; dorsal is up. A–E, The number of small HB9⁺ motor neurons in the ventromedial aspect of the spinal cord (B, C; ***p < 0.001) and of periventricular PCNA⁺ cells (D, E; **p < 0.01) are reduced by the heat-shock (hs) regimen depicted in A. F, Myc and PCNA (arrows) labeling is mutually exclusive in the ependymal layer. Scale bars, 50 μm.