Figure 9.

Coelectroporation of Lhx2 and Robo1 in the thalamus rescues the axon guidance phenotype of thalamocortical axons. A–C', Electroporated brains with Gfp (A, A'), Lhx2 (B, B'), and Lhx2+Robo1 (C, C'), respectively, showing the GFP-positive axons that were electroporated. Axons overexpressing Lhx2 and Robo1 follow a normal pathway toward the cortex as control experiments. D, Quantification of the data shown in A', B', and C' at the hypothalamic region as performed in Figure 3. Asterisks indicate significance at **p < 0.01 and ***p < 0.001, one-way ANOVA test with Tukey's post hoc analysis. E, Schematic diagram showing the dynamic regulation of Lhx2 protein in postmitotic thalamic neurons at rostrocaudal levels in relation to TCA pathfinding. Ncx, neocortex; Hyp, hypothalamus. Scale bars: (in A) A, B, C; 300 μm; (in A') A', B', C', 200 μm.