CaT2 released from the IP3-sensitive stores. A, Representative traces of Ca2+ transients in the presence of blockers. Atropine (1 μm, n = 5), pirenzepine (n = 3, data not shown), and mecamylamine (30 μm, n = 4) were applied to the bath solution. Heparin (1 mg/ml, n = 3) and ruthenium red (50 μm, n = 4) were in the pipette solution. EPSP of >∼20 mV amplitude by a single-shock synaptic stimulation was applied to evoke CaT2. B, Summary plot for the effect of blockers. **p < 0.01, compared with CCh.