Figure 3.

Overexpression of Lnk leads to decreased in vitro proliferation of SVZ NSPCs. A, Relative expression of Lnk in sorted GFP+ cells with or without retrovirally (RV) mediated Lnk-overexpression. Number (B) and size (C) of neurospheres formed from sorted RV-GFP and RV-GFP-Lnk transfected cells. D, Brightfield and fluorescence photomicrographs illustrating size difference between RV-GFP and RV-GFP-Lnk-transfected neurospheres. Percentage and photomicrographs of proliferating p-H3+ cells (E, F), and percentage of apoptotic TUNEL+ cells (G) out of the total number of Hoechst+ cells in sorted GFP+ and GFP+/LNK+ cells. Means ± SEM, n = 5 (B, C) and 4 (E, G). *p < 0.05, Student's unpaired t test. Scale bars: D, 50 μm; F, 10 μm.