Figure 7.

ProN expression reduces the spontaneous EPSC frequency. Representative traces (left) from whole-cell voltage-clamp recordings showing a higher frequency of sEPSCs in neurons transfected with N-cad (N-cad/GFP 4:1) compared with those transfected with ProN (ProN/GFP 4:1). Addition of factor Xa increases the frequency of spontaneous events in ProN-expressing neurons. Quantification of sEPSC frequency (right) shows a significant decrease in ProN-expressing neurons compared with N-cad (**p < 0.01; n = 11). Factor Xa treatment rescues sEPSC frequency in neurons transfected with mutant ProN (**p < 0.01; n = 9–11). Error bars indicate SEM.