| Kim et al. (2010) |
PCL/HA (Infused SDF1- and BMP7-loaded collagen) |
FDM |
In vivo |
22 male (12-week-old) Sprague-Dawley rats: 1 Rat’s dorsum subcutaneous pouches for human mandibular molar scaffolds, 2 right mandibular central incisor for rat central incisor teeth |
9 weeks |
A putative periodontal ligament and new bone regenerate at the interface of rat incisor scaffold with native alveolar bone by cell homing. |
| Lee et al. (2014) |
PCL/HA 100 um, 300 um, 600 um. |
FDM |
In vitro In vivo |
1 DPSCs, 2 PDLSCs, 3 ABSCs. The dorsum’s mid-sagittal plane for 10-week-old immunodeficient mice (Harlan) |
4 weeks |
DPSC-seeded multiphase scaffolds yield aligned PDL-like collagen fibers. The fibers inserted into bone sialoprotein-positive bone-like tissue and putative cementum matrix protein 1-positive/dentin sialophosphoprotein-positive dentin/cementum tissues. |
| Xue et al. (2013) |
Alginate/ gelatin |
Hydrogel extrusion |
In vitro |
hDPCs |
|
Self-defined shaped 3D constructs are printed and achieve the cell viability of 87%. |
| Jensen et al. (2014) |
PCL |
FDM |
In vitro |
hDPCs |
S3 weeks |
The HT-PCL scaffold promotes cell migration and osteogenic differentiation. |
| Rasperini et al. (2015) |
PCL |
SLS |
In vivo |
Clinical case on a periodontitis patient‘s canine. |
13 months |
The case demonstrated a 3-mm gain of clinical attachment and partial root coverage. However, the scaffold became exposed at the 13th month. |
| Cho et al. (2016) |
PCL, collagen I gel |
FDM |
Ex vivo |
PDLSCs seeded PCL was placed on tooth root surface defect. |
6 weeks |
The new mineralized tissue layer seen in BMP-7 treated samples expressed cementum protein 1 (CEMP1) |
| Jung, Lee & Cho (2016) |
PEG, PCL, cell-laden Alginate |
Hydrogel extrusion and FDM |
In vitro |
|
|
Multiple-layer bioprinting teeth was fabricated with a frame, two kinds of cell-laden hydrogel and a support. |
